Quoting Commentary for I Samuel 6:7

וְעַתָּ֗ה קְח֨וּ וַעֲשׂ֜וּ עֲגָלָ֤ה חֲדָשָׁה֙ אֶחָ֔ת וּשְׁתֵּ֤י פָרוֹת֙ עָל֔וֹת אֲשֶׁ֛ר לֹא־עָלָ֥ה עֲלֵיהֶ֖ם עֹ֑ל וַאֲסַרְתֶּ֤ם אֶת־הַפָּרוֹת֙ בָּעֲגָלָ֔ה וַהֲשֵׁיבֹתֶ֧ם בְּנֵיהֶ֛ם מֵאַחֲרֵיהֶ֖ם הַבָּֽיְתָה׃

Now therefore take and prepare you a new cart, and two milch kine, on which there hath come no yoke, and tie the kine to the cart, and bring their calves home from them.

Rashi

עלות means bringing up their young. Words of the same root are found in (Lamentations 2:11) “young children (עולל) and the sucklings”: (IsaiahLXV.20) “an infant (עול) of days”: (1 Samuel 6:7) “and two milch kine (עלות)”. old French enfantées.
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